Cloned (Comment) | Organism |
---|---|
gene lars, quantitative real-time PCR expression analysis | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Bos taurus | 5737 | - |
additional information | subcellular localization analysis | Bos taurus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-leucine + tRNALeu | Bos taurus | - |
AMP + diphosphate + L-leucyl-tRNALeu | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | A6QLR2 | Holstein dairy cows | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
connnective tissue | - |
Bos taurus | - |
epithelial cell | - |
Bos taurus | - |
mammary gland | LeuRS expression in mammary gland tissue is significantly higher during lactation compared to pregnancy. In pregnancy mammary gland tissue LeuRS showed no difference in the acinar buds and the connective tissue. While in lactating mammary gland tissue, most of LeuRS is expressed in the acinar buds with only a small amount detected in the connective tissue, quantitative real-time PCR expression analysis. the level of LeuRS expression is significantly higher in lactating mammary gland tissue than in pregnancy mammary gland tissue | Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-leucine + tRNALeu | - |
Bos taurus | AMP + diphosphate + L-leucyl-tRNALeu | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LARS | - |
Bos taurus |
Leucyl-tRNA synthetase | - |
Bos taurus |
LeuRS | - |
Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Bos taurus |
Organism | Comment | Expression |
---|---|---|
Bos taurus | the level of LeuRS expression is significantly higher in lactating mammary gland tissue than in pregnancy mammary gland tissue | additional information |
General Information | Comment | Organism |
---|---|---|
malfunction | siRNA-mediated knockdown of LeuRS leads to suppression of rapamycin (mTOR), p-mTOR, ribosomal protein S6 kinase 1 (S6K1), p-S6K1, beta-casein, sterol regulatory element binding-protein 1c (SREBP-1c), glucose transporter 1 (GLUT1), and cyclin D1 mRNA and protein expression. LeuRS knockdown reduces cell growth, the expression of lactation-associated proteins, and milk synthesis | Bos taurus |
physiological function | leucyl-tRNA synthetase regulates lactation and cell proliferation via mTOR signaling in dairy cow mammary epithelial cells, role of LeuRS as an intracellular L-leucine sensor for the mTORC1 pathway. LeuRS up-regulates the mTOR pathway to promote proliferation and lactation of dairy cow mammary epithelial cells (DCMECs) in response to changes in the intracellular leucine concentration. Treatment with L-leucine increases DCMECs viability and proliferation, as well as mammalian target of rapamycin (mTOR), p-mTOR, ribosomal protein S6 kinase 1 (S6K1), p-S6K1, beta-casein, sterol regulatory element binding-protein 1c (SREBP-1c), glucose transporter 1 (GLUT1), and cyclin D1 mRNA and protein expression via activity of enzyme LeuRS. Secretion of lactose and triglyceride are also increased. Effect of leucine on LeuRS to regulate cell growth and expression of proteins involved in mTOR signaling in DCMECs | Bos taurus |